Published on:
    Journal of Cardiovascular Disease Research, 2010; 1(3):152-157
    Original Article | doi:10.4103/0975-3583.70922

    Polymorphisms of methylenetetrahydrofolate reductase gene as the genetic predispositions of coronary artery diseases in eastern India

    Authors:

    Soujatya Dhar, Sumana Chatterjee, Saumitra Ray1,Anjanlal Dutta1, Bani Sengupta, Shila Chakrabarti

    Thalassemia Research Unit,

    1Departments of Medicine and Cardiology, Ramakrishna Mission Seva Pratishthan, Vivekananda Institute of Medical Sciences 99, Sarat Bose Road, Kolkata – 700 026, West Bengal, India

    Abstract:

    Background: Gene–environment interaction is an important aspect in the development of coronary artery disease (CAD). The mutation (677C-T) of methylenetetrahydrofolate reductase (MTHFR) gene results in a decrease of the enzyme activity that leads to mild hyperhomocysteinemia. Elevated plasma level of homocysteine has been recognized as an independent risk factor for cardiovascular disease. A case–control study was designed to assess whether the prevalence of some MTHFR gene polymorphisms have any role in the development of CAD. Materials and Methods: The study included unrelated 217 cases with CAD and 255 healthy controls. DNA was extracted from peripheral blood. MTHFR genotypes were identifi ed by seeing the presence or absence of 677C->T mutation obtained by PCR followed by Hinf1 restriction digestion. Multiple logistic regression analysis was carried out to fi nd association between studied genotypes and lifestyle as well as biochemical risk factors. Results: The T allele was found to be associated with the disease. Signifi cant associations were found with smoking, hypertension, diabetes, and family history of CAD. Conclusion: The results indicate that MTHFR 677C-T polymorphism has signifi cant association with CADs in the population of eastern India.

    Key words: Allele, case–control, methylenetetrahydrofolate reductase, multiple logistic regression, polymorphism.